Premium
Should we be counting immunogold marker particles on cell surfaces with the SEM?
Author(s) -
Harven E.,
Soligo D.,
Christensen H.
Publication year - 1987
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1987.tb01339.x
Subject(s) - immunogold labelling , colloidal gold , scanning electron microscope , monoclonal antibody , antigen , electron microscope , conjugate , antibody , chemistry , colloidal particle , biophysics , biology , materials science , nanotechnology , colloid , nanoparticle , optics , immunology , physics , mathematics , mathematical analysis
SUMMARY The lymphocyte in Fig. 1 was incubated with a 1/10 dilution of a murine monoclonal antibody (Leu‐1) directed against a surface antigen expressed on circulating human T‐cells. It was labelled with a colloidal gold/immunoglobulin conjugate and examined with the scanning electron microscope (SEM) in the backscattered electron imaging (BEI) mode. It was easy to count the number of gold particles seen on this cell; actually, we counted 471 of them. For biologists, is this number relevant? We will discuss the principal factors involved in answering this question.