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Scanning acoustic microscopy visualizes cytomechanical responses to cytochalasin D
Author(s) -
BereiterHahn Jürgen
Publication year - 1987
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1987.tb01324.x
Subject(s) - phalloidin , cytochalasin , acoustic microscopy , cytochalasin d , scanning electron microscope , microscopy , biophysics , actin , xenopus , fluorescence microscope , chemistry , materials science , optics , fluorescence , biology , cytoskeleton , physics , cell , biochemistry , composite material , gene
SUMMARY The reactions of XTH‐2 cells (line derived from Xenopus laevis tadpole hearts) to cytochalasin D (CD) were followed using scanning acoustic microscopy (SAM) at 0.9 GHz, fluorescence and electron microscopy. The first reaction to CD which can be detected by SAM is a loss of image contrast, indicating a decrease in acoustic impedance of about 30%. Based on structural changes revealed by staining of actin with TRITC‐phalloidin, and taking theoretical considerations into account, a relationship between impedance decrease and tension in the actin fibrillar system is deduced.