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Averaging of periodic images using a microcomputer
Author(s) -
Squire John M.,
Luther Pradeep K.,
Agnew Gerard D.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb04284.x
Subject(s) - microcomputer , fourier transform , transformation (genetics) , basis (linear algebra) , computer science , computer vision , space (punctuation) , computer graphics (images) , image (mathematics) , artificial intelligence , fast fourier transform , image processing , interface (matter) , algorithm , mathematics , mathematical analysis , geometry , telecommunications , chip , biochemistry , chemistry , bubble , maximum bubble pressure method , parallel computing , gene , operating system
SUMMARY A system based on a commercial microcomputer and a commercial image‐capturing TV interface is described which carries out both real space and conventional Fourier transform‐based image averaging procedures. Various real space manipulations are described for handling images with either one‐dimensional or two‐dimensional periodic features. In certain cases, real space averaging procedures have many advantages and are recommended to those not familiar with the mathematics of methods based on Fourier transformation. In other cases Fourier transformation processes are necessary and these also have been implemented on the microcomputer. They can be used to determine periodicities, to produce two‐dimensional average images or as a basis for three‐dimensional reconstruction. Examples showing the principles of application of these methods use appropriate images from electron micrographs of muscle and collagen.