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Visualization of biological specimens by cryoHVEM
Author(s) -
Fotino Mircea
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02785.x
Subject(s) - penetration (warfare) , microscopy , visualization , materials science , electron microscope , high resolution , biological specimen , ultrastructure , fixation (population genetics) , microscope , biomedical engineering , chemistry , optics , computer science , biology , anatomy , geology , remote sensing , data mining , medicine , physics , biochemistry , operations research , gene , engineering
summary This article describes the operation and the characteristics of cryoHVEM imaging of biological specimens using a top‐entry cryostage. The procedure for inserting frozen specimens into the microscope column is also presented. Whole mounts were thus observed under optimal imaging conditions by combining: (i) fixation by fast freezing for structure preservation without exposure to chemicals, (ii) observation in the hydrated (frozen) state or in the dried state without exposure to the atmosphere after the initial fixation by freezing, and (iii) ultrastructural visualization with the key imaging factors of resolution, penetration and beam‐induced damage at their best by high‐voltage electron microscopy.