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Simple SEM stereophotogrammetric method for three‐dimensional evaluation of features on flat substrates
Author(s) -
Boyde A.,
Howell P. G. T.,
Franc F.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02782.x
Subject(s) - projection (relational algebra) , microcomputer , computer science , computer vision , simple (philosophy) , substrate (aquarium) , surface (topology) , artificial intelligence , computer graphics (images) , biomedical engineering , materials science , geology , mathematics , algorithm , engineering , geometry , telecommunications , chip , philosophy , oceanography , epistemology
summary A semi‐automated method was developed for the measurement of the volume of a calcified tissue substrate which can be resorbed by single, isolated resorptive cells (osteoclasts). The procedure would, however, be applicable to the study of any pit in, or projection from, a flat surfaced specimen. Stereo‐pair images are recorded with an SEM. 3‐D co‐ordinates of relevant surface features are derived using a stereo‐comparator which outputs data directly to a microcomputer. Algorithms adopted have been chosen to give the best compromise between accuracy and speed of data acquisition.