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The use of gold markers in immunocytochemical studies of microbiological organisms: a review
Author(s) -
Beesley Julian E.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02776.x
Subject(s) - immunocytochemistry , colloidal gold , gold standard (test) , stain , embedding , antigen , biology , nanotechnology , microbiology and biotechnology , computational biology , computer science , materials science , pathology , staining , immunology , medicine , artificial intelligence , genetics , radiology , nanoparticle
SUMMARY Colloidal gold probes have revolutionized electron immunocytochemistry and are now used extensively for microbiological studies. The gold probes can be applied in one of several methods to gain valuable information from the sample. The pre‐embedding technique, the post‐embedding technique, the immunonegative stain technique, the immunoreplica technique and the immunofreeze‐fracture technique, are each described and their applications in microbiology, for diagnosis, for the detection of the site of antigen synthesis in host cells, for vaccine production and for genetic engineering are reported.