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Light microscopical detection of single 5 and 20 nm gold particles used for immunolabelling of plasma membrane antigens with silver enhancement and reflection contrast
Author(s) -
Hoefsmit E. C. M.,
Korn C.,
Blijleven N.,
Ploem J. S.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02774.x
Subject(s) - microscopy , antigen , monoclonal antibody , chemistry , reflection (computer programming) , membrane , materials science , biophysics , antibody , optics , biology , biochemistry , immunology , physics , computer science , programming language
SUMMARY To detect plasma membrane antigens, cytocentrifuge preparations of the macrophage‐like cell line P388D1 were incubated with monoclonal antibodies and labelled with 5 and 20 nm gold particles conjugated to immunoglobulins or protein A. The 20 nm, but not the 5 nm, particles could be observed by reflection‐contrast light microscopy. Single 5 nm particles, however, were clearly demonstrable with silver contrast enhancement, both in absorption contrast and in reflection contrast. The method proved to be compatible with cytochemical methods such as acid phosphatase.