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Post‐embedding cytochemistry with gold‐labelled reagents: a review
Author(s) -
Roth J.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02771.x
Subject(s) - cytochemistry , glycoconjugate , colloidal gold , staining , reagent , lectin , chemistry , electron microscope , antigen , microscopy , biochemistry , nanotechnology , biology , materials science , pathology , optics , immunology , medicine , physics , nanoparticle , enzyme , organic chemistry
SUMMARY The detection of antigens and glycoconjugates with the protein A–gold and the lectin–gold techniques, respectively, is reviewed. Special attention is directed to the necessary conditions for fixation and embedding as well as to the staining procedures of tissue sections for light and electron microscopy.