Premium
Developments of new Lowicryl® resins for embedding biological specimens at even lower temperatures
Author(s) -
Acetarin JeanDominique,
Carlemalm Eric,
Villiger Werner
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02766.x
Subject(s) - materials science , hardening (computing) , biological materials , polymerization , composite material , polymer chemistry , polymer , biomedical engineering , medicine , layer (electronics)
SUMMARY Two new Lowicryl resins have been developed for embedding biological materials at temperatures down to 210K (hydrophilic K11M) and to 190K (hydrophobic HM23). They have similar properties to Lowicryl K4M and HM20. The new resins were first tested for low temperature applications by the ‘progressive lowering of temperature’ procedure and this shows that the low viscosity of K11M and HM23 is favourable for the infiltration of biological specimens. Hardening is achieved through photo‐polymerization at these lower temperatures. These properties make K11M and HM23 suitable for cryosubstitution of rapidly frozen material and it is speculated that the preservation of antigenicity may be further improved.