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Electron microscopic study of 5SrRNA crystals from Thermus thermophilus HB8
Author(s) -
Morikawa K.,
Fujiyoshi Y.,
Ishizuka K.,
Sugiyama J.,
Kawakami M.,
Takemura S.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02761.x
Subject(s) - thermus thermophilus , crystallography , uranyl acetate , crystal (programming language) , electron microscope , thermophile , crystal structure , materials science , lattice (music) , negative stain , chemistry , optics , physics , biochemistry , enzyme , escherichia coli , computer science , acoustics , gene , programming language
SUMMARY Several types of crystals were grown from 5SrRNA, which was purified from the highly thermophilic bacterium, Thermus thermophilus HB8. Crystal lattice parameters were determined by X‐ray and electron diffraction. One type of crystal was suitable for electron microscopy after staining with uranyl acetate. Two projections derived from micrographs taken at different tilt angles were processed for image analysis. This result enabled us to deduce the arrangement of molecules within the crystal lattice.