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Differential staining of dead and dying embryonic cells with a simple new technique
Author(s) -
MartínPartido G.,
Alvarez I. S.,
RodríguezGallardo L.,
Navascués J.
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02742.x
Subject(s) - staining , differential staining , toluidine , safranin , glutaraldehyde , embryonic stem cell , biology , pathology , microbiology and biotechnology , chemistry , anatomy , biochemistry , medicine , gene
SUMMARY A new staining technique with toluidine blue and safranin is described which yields satisfactory results in histological sections of chick embryo tissue fixed in a mixture of glutaraldehyde and formaldehyde and embedded in Spurr's resin. In zones of embryonic cell death, this technique allows the blue‐staining of dying cell pyknotic nuclei to be clearly distinguished from red‐staining healthy cells. The possible factors underlying this differential staining of pyknotic nuclei are discussed.