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Microscopic observation and video recording of cellular response to sudden changes in environment
Author(s) -
Eskelinen Sinikka,
Coakley W. Terence
Publication year - 1986
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1986.tb02706.x
Subject(s) - video recording , computer science , computer graphics (images)
SUMMARY A method to record responses of single cells to rapid changes in their environment is described. A rectangular glass microcapillary is filled with the cell suspension under investigation and placed under a light microscope. With the aid of a micromanipulator a smaller microcapillary with a narrowed tip, which was still over 10 times wider than the cell diameter, is driven into the larger, cell containing, microcapillary. The environment of the cells is changed by injecting a new medium via the smaller capillary and the cell responses are recorded using a video recording system. The usefulness of the system has been examined by studying the time course of the osmotic swelling of erythrocytes after water injection and the shape transformations caused by lysophosphatidylcholine.