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Automatic analysis of the motion of microorganisms
Author(s) -
Gualtieri P.,
Colombetti G.,
Lenci F.
Publication year - 1985
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1985.tb04661.x
Subject(s) - computer vision , computer science , motion picture , microscope , artificial intelligence , video camera , computer graphics (images) , professional video camera , line (geometry) , computer image , image processing , frame (networking) , optics , image (mathematics) , physics , telecommunications , mathematics , geometry , high definition television
SUMMARY An automatic, interpretative, off‐line computer‐television‐microscope system for studying the behaviour of microorganisms is presented. The equipment should be available even in relatively modestly equipped laboratories. The movement of microorganisms freely swimming in the chamber of a depression slide is recorded on a video tape with the aid of a microscope and an infrared TV camera. The images are acquired by means of a frame grabber in an image controller and elaborated by a PDP 11/23 computer. The procedures used for data processing are discussed in detail. Trajectories reconstructed by the computer are shown to be exactly superimposed to the real ones, and the motion parameters can be easily determined.