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Theoretical calculation of layer geometry in rotary shadowed models of membrane‐associated particles
Author(s) -
Landmann L.,
Roth J.
Publication year - 1985
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1985.tb02638.x
Subject(s) - ellipsoid , cylinder , layer (electronics) , membrane , spherical geometry , materials science , spheres , geometry , mechanics , basis (linear algebra) , physics , chemistry , nanotechnology , mathematics , biochemistry , astronomy
SUMMARY The geometry of the evaporated metal layer on models of membrane‐associated particles has been calculated theoretically and the respective density profiles have been estimated. The models were a hollow cylinder, a hemisphere and a rotary ellipsoid substituting the freeze‐fracture appearance of gap junction connexons and globular integral membrane proteins respectively. These calculations may be used as a help for choosing an optimal compromise between shadowing angle and layer thickness. They furthermore provide a basis for assaying the extent of shadowing artefacts such as decoration and lead to a more accurate interpretation of rotary shadowed structures.