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Laser scanning microscopy of surface spread polytene chromosomes
Author(s) -
Kalisch W.E.,
Whitmore T.,
Siegel A.
Publication year - 1985
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1985.tb02579.x
Subject(s) - polytene chromosome , microscopy , biology , optics , differential interference contrast microscopy , laser , chromosome , biophysics , physics , genetics , gene
SUMMARY A new type of a Laser Scan Microscope (Zeiss) was used for the analysis of the band‐interband pattern of polytene chromosomes in Chironomus. In contrast to the previously used techniques of transmission light and electron microscopy, we used differential interference contrast (DIC) in incident light to depict the pattern. Instead of using common squash preparations, we carried out this investigation with surface spread polytene (SSP) chromosome preparations of salivary glands. The combination of techniques used enabled a more detailed light microscopic presentation of polytene structures in individual preparations than conventional techniques used so far for chromosome mapping.