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Polyethylene glycol embedding of plant tissues for transmission electron microscopy
Author(s) -
Hawes C. R.,
Horne J. C.
Publication year - 1985
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1985.tb02559.x
Subject(s) - polyethylene glycol , nucleoplasm , organelle , transmission electron microscopy , staining , cytoplasm , polyethylene , microscopy , electron microscope , chemistry , materials science , biophysics , nanotechnology , biology , biochemistry , optics , physics , organic chemistry , nucleolus , genetics
SUMMARY The use of high molecular weight polyethylene glycol as an embedding medium for the production of resin‐free sections of plant cells is described. Specimens can be observed at a range of accelerating voltages, are high in contrast and require no heavy metal staining. All the major organelles appear intact and a three‐dimensional network of filaments in the cytoplasm and nucleoplasm is reported.