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A methodological basis for SEM autoradiography: biosynthesis and radioligand binding
Author(s) -
Cheng G.,
Hodges G. M.,
Trejdosiewicz L. K.
Publication year - 1985
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1985.tb02555.x
Subject(s) - scanning electron microscope , radioligand , receptor , biophysics , chemistry , electron microscope , resolution (logic) , cell , biosynthesis , high resolution , radioligand assay , biochemistry , biology , enzyme , optics , physics , geology , computer science , remote sensing , artificial intelligence
SUMMARY A method is described for scanning electron microscope (SEM) autoradiography whereby preservation of high resolution cell surface details is retained together with degelatination of the emulsion without gross loss or redistribution of silver grains. This method should provide a convenient medium‐sized marker for SEM (using secondary, backscattered electron and X‐ray imaging) topographic studies of biosynthesized molecules, and of cell surface receptors and antigens, using indirect or direct labelling procedures with radio‐labelled ligands.