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A simple and inexpensive liquid helium cooled ‘slam freezing’ device
Author(s) -
Heath I. Brent
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb04650.x
Subject(s) - simple (philosophy) , liquid helium , block (permutation group theory) , substitution (logic) , helium , materials science , water cooled , computer science , nuclear engineering , chemistry , mechanical engineering , engineering , water cooling , mathematics , philosophy , geometry , organic chemistry , epistemology , programming language
SUMMARY A liquid helium cooled copper block device has been designed for the rapid freezing of biological material. The apparatus differs from previous designs in being easily constructed from readily available components. It incorporates a novel and simplified specimen carrying system. Construction of this apparatus is possible for any laboratory and makes the advantages of freeze substitution reliably available for many cell types at minimal cost.