Premium
Double‐axis rotary replication for deep‐etching
Author(s) -
Shibata Y.,
Arima T.,
Yamamoto T.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb02552.x
Subject(s) - replication (statistics) , etching (microfabrication) , simple (philosophy) , vertical axis , materials science , computer science , geometry , mechanical engineering , engineering drawing , nanotechnology , mathematics , engineering , philosophy , epistemology , statistics , layer (electronics)
SUMMARY We have developed a simple new method of rotary shadowing, double‐axis rotary replication, for observing three‐dimensional structures in deep‐etched, rapid‐frozen tissues. The technical details of this method are described and compared with the conventional fixed‐angle rotary shadowing procedure.