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Representation of three‐dimensionally reconstructed objects in electron microscopy by surfaces of equal density
Author(s) -
Radermacher M.,
Frank J.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb02547.x
Subject(s) - representation (politics) , surface (topology) , particle (ecology) , boundary (topology) , computer science , electron microscope , boundary representation , microscopy , computer vision , visualization , artificial intelligence , surface reconstruction , optics , computer graphics (images) , physics , geometry , mathematics , geology , mathematical analysis , oceanography , politics , political science , law
SUMMARY A surface representation algorithm has been developed to visualize the results of three‐dimensional reconstructions from projections in electron microscopy. It produces an easily recognizable image of the reconstructed particle and facilitates comprehension of the three‐dimensional structure. Thus building of physical models can be avoided in many cases. If such a model is needed the algorithm can be used to establish the correct particle boundary in advance. Some further effort has been made to represent long fibres and visualize inner structures of objects, which are obscured by outer high‐density regions. Shading may be used to enhance the depth perception from single views.