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Measurement of cellular elastic properties by acoustic microscopy
Author(s) -
Hildebrand J. A.,
Rugar D.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb02518.x
Subject(s) - acoustic microscopy , microscope , scanning acoustic microscope , materials science , microscopy , acoustic impedance , elasticity (physics) , optical microscope , cytoplasm , acoustic attenuation , attenuation , acoustics , optics , scanning electron microscope , chemistry , physics , ultrasonic sensor , composite material , biochemistry
SUMMARY The acoustic microscope is used to investigate the elastic properties of living biological cells. A quantitative model is developed relating acoustic microscope image contrast to cellular elastic properties. Cytoplasmic acoustic attentuation is measured by focusing the acoustic microscope on the surface of the underlying substrate. Cytoplasmic acoustic impedance is measured by focusing the acoustic microscope on the top surface of the cell. The model allows the acoustic microscope to give quantitative information about cellular elasticity on a subcellular scale.