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Impregnation of biological material by ZnI 2 ‐OsO 4 , KI‐OsO 4 and NaI‐OsO 4 mixtures for electron microscopic observations: Chemical interpretation of the reaction
Author(s) -
Carrapiço F.,
MadalenaCosta F.,
Pais Maria Salomé S.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb02511.x
Subject(s) - iodide , chemistry , iodine , osmium , redox , electron , osmium tetroxide , analytical chemistry (journal) , inorganic chemistry , organic chemistry , electron microscope , physics , catalysis , quantum mechanics , ruthenium , optics
SUMMARY Alkaline iodide‐osmium tetroxide mixtures were used in an electron microscopic study of plant cells and gave better results in the preservation of the cell structure in contrast to the cell structure observed when ZnI 2 ‐OSO 4 mixtures are used. The electron density observed in the reactive sites of the cell by OsO 4 ‐iodide mixtures is controlled by the presence and oxidation state of iodine that acts as a regulator of the potential redox of the reaction of OsO 4 with the biological material.