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An automatic pulsed laser microfluorometer with high spatial and temporal resolution
Author(s) -
Docchio F.,
Ramponi R.,
Sacchi C. A.,
Bottiroli G.,
Freitas I.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb02504.x
Subject(s) - laser , photomultiplier , microscope , optics , nitrogen laser , signal (programming language) , fluorescence , image resolution , dye laser , microscopy , materials science , laser induced fluorescence , fluorescence microscope , temporal resolution , resolution (logic) , wavelength , physics , computer science , artificial intelligence , detector , programming language
SUMMARY The paper describes an automatic pulsed laser microfluorometer with high spatial and temporal resolution, developed in our laboratories. The instrument consists of: (i) a nitrogen‐laser‐pumped dye‐laser for the excitation of the fluorescence, (ii) a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, (iii) filters or monochromators to select the output wavelength, (iv) a fast photomultiplier tube to detect the signal, and (v) a dual time‐scale microprocessor‐controlled signal averager for the acquisition and processing of the signal. Examples are given that show the potential of the time‐resolved fluorescence microscopy in studying, quantitatively and qualitatively, the properties of fluorescent molecules.