Premium
The role of carbamino compounds in histological staining
Author(s) -
Cook D. J.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb00548.x
Subject(s) - staining , methylene blue , eosin , eosin y , h&e stain , chemistry , methylene , nuclear chemistry , biochemistry , pathology , organic chemistry , medicine , photocatalysis , catalysis
SUMMARY The effects of high CO 2 concentration on tissue staining with buffered eosin, methylene blue and azure A were investigated to determine if carbamino compound formation could alter dye binding. Only in the case of eosin staining were the differences in results with high and low CO 2 concentrations compatible with the formation of carbamino compounds. The differences observed with basic dyes were statistically significant but could not be accounted for by carbamino compound formation.