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Extraction of substances during glutaraldehyde fixation of plant cells
Author(s) -
Coetzee J.,
Merwe C. F.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb00515.x
Subject(s) - glutaraldehyde , extraction (chemistry) , tris , chemistry , chromatography , phosphate , fixation (population genetics) , phosphate buffered saline , nuclear chemistry , biochemistry , gene
SUMMARY Extraction of Ca, Mg, reducing sugars, amino acids and protein was monitored during fixation of bean leaves. Fixatives included 0.5–5% glutaraldehyde in water at pH 3.4 and at pH 7.2 and phosphate, citrate, Na‐cacodylate, tris, glycyleglycine, collidine and four ‘Good’ buffers, all with 2.5% glutaraldehyde. Least extraction was found in phosphate buffered glutaraldehyde. The optimal glutaraldehyde concentration is 2–3%. This causes least extraction of substances.