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Automatic analysis of G‐banded human chromosomes by fast scanning microscope photometry. I. Basic principles and computer control
Author(s) -
Wall W. J.,
Butler L. J.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb00498.x
Subject(s) - photometry (optics) , microscope , photometer , optics , microscopy , computer science , materials science , physics , computer vision , stars
SUMMARY A high resolution scanning microscope photometry system has been developed for use with standard G‐banded chromosome preparations. Various alternative scanning methods and their limitations are reviewed. The computer algorithms which we use are described for the manipulation of the digitized image as produced by the microscope photometer; these include noise reduction and contrast enhancement as well as altering the orientation of the chromosome.