Premium
The current status of fixation for electron microscopy: A review
Author(s) -
Bullock Gillian R.
Publication year - 1984
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1984.tb00458.x
Subject(s) - fixative , fixation (population genetics) , electron microscope , microscopy , immunocytochemistry , biology , biophysics , microbiology and biotechnology , chemistry , pathology , biochemistry , cytoplasm , optics , physics , medicine , endocrinology , gene
SUMMARY Chemical fixation of cells has been used extensively in different fields of electron microscopy. The type of fixation can be selected to maximize retention of different cell constituents where morphology is of prime interest. For techniques such as immunocytochemistry and autoradiography, a compromise between morphology and retention of antigenicity or radiolabel has to be reached. By careful choice of fixative, buffer and other physical parameters, optimum results can be obtained for both animal and plant tissues. Emphasis has been put on more recent developments in technique.