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Marking the origin of coordinates in high‐resolution light‐microscopy scanning
Author(s) -
Bahr Gunter F.,
Boccia Joseph A.
Publication year - 1983
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1983.tb04264.x
Subject(s) - resolution (logic) , optics , microscope , high resolution , computer science , microscopy , materials science , computer vision , computer graphics (images) , artificial intelligence , geology , remote sensing , physics
SUMMARY In high‐resolution image analysis, it is often desirable to return to a chosen cell after it has been restained or subjected to histochemical procedures. The reading of the vernier on the microscope stage is too coarse for relocating of non‐distinct single cells, because the accuracy, determined by visual interpolation, is limited, at best, to 1/20th of a millimetre, or 50 μm. When one works with haematologic samples, e.g. lymphocytes, the precision of relocating a cell has to be better than 50 μm; i.e. the cell should reappear near the centre of the visual field of a x 100 oil‐immersion objective. We describe a simple device by means of which distinct marks can be made on a slide with specimen (but before coverslipping) and will provide suitable origins for a coordinate system that will cover the entire preparation.