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The complex‐shaped ‘perforated’ synapse, a problem in quantitative stereology of the brain
Author(s) -
Groot Didima M. G. De,
Bierman Egbertus P. B.
Publication year - 1983
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1983.tb04260.x
Subject(s) - stereology , synapse , neuroscience , biomedical engineering , materials science , computer science , biology , medicine , endocrinology
SUMMARY Failure to appreciate the consequences for stereological work of the simultaneous presence of complex‐shaped perforated and disc‐like non‐perforated synapses in brain tissue results in underestimation of synaptic profile length and overestimation of synaptic density when measured in randomly selected ultrathin E‐PTA slices. This problem can be solved by using serial slices and a calculation method which makes no assumptions about synaptic size and shape. A three‐dimensional reconstruction is unnecessary.