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A high‐resolution staining method for examination of mammalian cell surfaces in the light microscope
Author(s) -
Junker Steffen
Publication year - 1983
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1983.tb04249.x
Subject(s) - microscope , staining , electron microscope , resolution (logic) , microscopy , scanning electron microscope , materials science , optics , optical microscope , high resolution , biology , composite material , physics , computer science , genetics , artificial intelligence , remote sensing , geology
SUMMARY A simple light‐microscopic method for the examination of fine details on cell surfaces is described. When cell specimens on platinum‐coated glass or plastic surfaces are stained with 3 ,3′‐diaminobenzidine tetrahydrochloride and hydrogen peroxide, fine cytoplasmic processes that cannot be detected using conventional staining techniques for the light microscope are revealed. It is suggested the new technique be applied instead of the scanning electron microscope when the limited resolution of the light microscope is sufficient.