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Standards for quantitative energy dispersive X‐ray microanalysis of biological cryosections: validation and application to studies of myocardium
Author(s) -
Hagler Herbert K.,
Lopez Linda E.,
Flores Jesse S.,
Lundswick Rebecca J.,
Buja L. Maximilian
Publication year - 1983
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1983.tb04248.x
Subject(s) - microanalysis , elemental analysis , analytical chemistry (journal) , chemistry , materials science , biological system , chromatography , inorganic chemistry , biology , organic chemistry
SUMMARY The preparation of a comprehensive set of gelatin‐glycerol‐salt standards for quantitation of diffusible ions in ultrathin cryosectioned tissue is described. This set of standards serves two basic functions. The first is to calibrate the X‐ray analytical system so that peak‐to‐continuum ratios obtained from test specimens may be converted to elemental concentrations. The second is to provide a known set of elemental concentrations which can be used to check the performance of the preparative and analytical steps involved in thin section biological X‐ray microanalysis. The application of these standards and techniques for cryoultramicrotomy and analysis of myocardial tissue also is described.