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A simple and efficient method for objective discrimination between pseudostratified, metaplastic and dysplastic nasal epithelium
Author(s) -
Boysen M.,
Marton P. F.,
Pilström L.,
Solberg L. A.,
Torp T.
Publication year - 1983
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1983.tb04203.x
Subject(s) - epithelium , basal (medicine) , transversal (combinatorics) , basement membrane , consistency (knowledge bases) , pathology , mathematics , stratified squamous epithelium , anatomy , biology , combinatorics , medicine , geometry , mathematical analysis , insulin , endocrinology
SUMMARY Morphologic features may sometimes be interpreted differently by different pathologists. By introducing objective methods in the histological evaluation the subjectivity of the judgement is reduced and a higher degree of consistency achieved. In the present study three linear parameters of nasal epithelial basal cells: the largest transversal nuclear diameter, the sum of the longitudinal and transversal nucleolar axes and the basal cell width, i.e. the width of the attachment face to the basement membrane, were classified by means of three‐class rulers. From this classification weighted indices were made which were then given equal weight and transformed, so that when added up their sum, the joint index ( Q ) to the largest possible extent was consistent with pseudostratified/stratified cuboidal, stratified squamous and dysplastic epithelium when Q ≤ 1, 1 < Q ≤ 2 and Q > 2 respectively. Tested prospectively we found 91% agreement between the morphometric and histological classification. The method presented is extremely simple and rapid to perform, and appears to have diagnostic potential in distinguishing various types of epithelial alterations of the nasal mucosa. The method may also be useful in approaching similar problems in other epithelia.