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Calibration of transmission electron microscopic mass determination using objects with known mass distribution
Author(s) -
Linders P. W. J.,
Stols A. L. H.,
Stadhouders A. M.
Publication year - 1983
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1983.tb04201.x
Subject(s) - calibration , magnification , mass distribution , calibration curve , optics , transmission (telecommunications) , analytical chemistry (journal) , materials science , chemistry , physics , chromatography , mathematics , statistics , computer science , quantum mechanics , detection limit , galaxy , telecommunications
SUMMARY An improved calibration procedure is described for dry mass determination methods, that are based on microdensitometry of transmission electron micrographs. Polystyrene latex spheres, that are supposed to have a homogeneous mass distribution, are used for the calibration procedure. By computer fitting of the theoretically expected mass distribution to the distribution measured in a line through the centre of a sphere, a mass calibration factor is obtained. The number of measurements required for a predetermined calibration accuracy can be decreased considerably as compared to other calibration methods. Accurate calibration and reproducible adjustment of the electron optical magnification are required.