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Incident light microscopy of normal and transformed cultured fibroblasts stained with Coomassie blue R250
Author(s) -
Paddock Stephen W.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00453.x
Subject(s) - coomassie brilliant blue , cytoskeleton , microscopy , immunofluorescence , staining , blue light , indirect immunofluorescence , microbiology and biotechnology , phase contrast microscopy , chemistry , pathology , biology , optics , cell , medicine , biochemistry , antibody , physics , immunology
SUMMARY Incident light microscopy of fibroblasts stained with Coomassie blue R250 results in images of the cytoskeleton with a similar high contrast to those obtained by specific immunofluorescence microscopy. This technique is especially useful for the routine visualization of the cytoskeleton of transformed cells.