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The simultaneous use of Hanks' and Hepes buffers in the preparation of human leucocytes for SEM observation
Author(s) -
Lalague E. D.,
Haviernick S.,
Corvellec M.R.,
Pelletier L.,
Derghazarian C.,
Cousineau G. H.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00427.x
Subject(s) - hepes , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology
SUMMARY Leucocytes were isolated from the peripheral blood of normal individuals and prepared for SEM observation. The addition of the buffer Hanks and Hepes to the glutaraldehyde fixative was found to maintain the initial ambient pH throughout cell manipulation, and to allow satisfactory preservation of surface architecture.