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A new technique for perfusion fixation and contrast enhancement of foetal lamb myocardium for electron microscopy
Author(s) -
Dae Michael W.,
Heymann Michael A.,
Jones Albert L.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00426.x
Subject(s) - tannic acid , perfusion , fixation (population genetics) , electron microscope , microscopy , pathology , biomedical engineering , chemistry , medicine , cardiology , biochemistry , optics , physics , organic chemistry , gene
SUMMARY A technique of perfusion fixation following elective diastolic cardiac arrest was developed to obtain excellent preservation of foetal lamb hearts for morphological studies with electron microscopy. A technique was developed to markedly enhance the contrast of embedded material by treating thin sections with tannic acid prior to lead staining. This technique has been particularly useful for quantitative morphometric analysis.