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An intercellular network artefact in glycol methacrylate dehydrated neonatal cartilage
Author(s) -
Shepard Nora,
Mitchell Nelson,
Harrod John
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00424.x
Subject(s) - glutaraldehyde , glycosaminoglycan , methacrylate , cartilage , dehydration , transmission electron microscopy , chemistry , materials science , anatomy , biochemistry , biology , nanotechnology , chromatography , organic chemistry , polymerization , polymer
SUMMARY A striking network thought to consist of glycosaminoglycan in neonatal cartilage has recently been reported following glycol methacrylate (GMA) dehydration and embedding. This study attempted to learn why the network was not seen in other preparations previously reported. Comparative light and transmission electron microscopy of the network structures was carried out to determine their biological significance. We have shown the networks to be reproducible artefact, attained only following GMA dehydration of glutaraldehyde fixed neonatal cartilage.