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Point projection imaging of unstained ferritin molecules on tungsten *
Author(s) -
Panitz J. A.,
Ghiglia D. C.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00420.x
Subject(s) - ferritin , projection (relational algebra) , tungsten , molecule , microscopy , materials science , electron microscope , micrograph , electron micrographs , chemistry , optics , crystallography , physics , scanning electron microscope , computer science , biochemistry , algorithm , organic chemistry , metallurgy
SUMMARY Point‐projection microscopy has been used to obtain the first images of closely spaced (but isolated) unstained ferritin molecules on a tungsten substrate. The microscopy, in conjunction with a digital processing algorithm, produces images which display a striking three‐dimensional quality usually associated with scanning electron micrographs. From these images, an apoferritin diameter of 13 nm has been deduced. This is identical to the diameter of apoferritin obtained from a reconstruction of the molecule using X‐ray coordinate data, but almost 70% larger than that deduced from TEM images of ferritin in which only its iron rich core can be seen.