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Glycol methacrylate embedding of bone and cartilage for light microscopic staining
Author(s) -
Cole Madison B.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00406.x
Subject(s) - methacrylate , cartilage , staining , aqueous solution , polymerization , solvent , microscopy , materials science , chemistry , biomedical engineering , anatomy , pathology , polymer , composite material , biochemistry , biology , organic chemistry , medicine
SUMMARY A method is described for embedding bone and cartilage in glycol methacrylate (GMA) for light microscopy. Dehydration‐infiltration of the hard tissue is with aqueous GMA solutions minimizing solvent and dehydration artefact, and polymerization is by UV light in the cold to minimize thermal damage. Over fifty stains, enzyme localizations and related histochemical methods for 0·5–3·0 μm thick sections of GMA embedded tissue are listed. The increased resolution plus the localization of cellular and extracellular chemical moieties is now easier and more accurate providing an improved method for the study of the musculo‐skeletal system by light microscopic histochemistry.