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The correlation averaging of a regularly arranged bacterial cell envelope protein
Author(s) -
Saxton W. O.,
Baumeister W.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00405.x
Subject(s) - envelope (radar) , resolution (logic) , cell envelope , crystallinity , symmetry (geometry) , crystallography , correlation , electron micrographs , optics , chemistry , physics , biological system , materials science , biology , mathematics , computer science , electron microscope , artificial intelligence , geometry , biochemistry , telecommunications , radar , escherichia coli , gene
SUMMARY An adaptation of the ‘correlation averaging’ method is described which allows reliable and almost fully automatic image averaging in the case of near‐periodic structures notwithstanding the presence of substantial crystal imperfections; methods for assessing resolution and symmetry without reliance on crystallinity are also discussed. Electron micrographs of negatively stained and rotary shadowed preparations of the HPI‐layer protein from the cell envelope of Micrococcus radiodurans have been averaged using the method, and the projected structure is described to a resolution of about 1·9 nm.