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New instruments which facilitate rapid freezing at 83 K and 6 K
Author(s) -
Escaig Jacques
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00379.x
Subject(s) - thermocouple , immersion (mathematics) , copper , materials science , liquid helium , propane , helium , composite material , metallurgy , chemistry , mathematics , organic chemistry , pure mathematics
SUMMARY We have developed three instruments to freeze different biological specimens for various purposes in the easiest and most economical way: a punching device, a rapid immersion device and a device to freeze the specimens against a copper block cooled by liquid helium. Cooling rate measurements are made by a thin flat thermocouple. Freezing against a copper block cooled down to 4 K gives cooling rates 3–5 times higher than freezing by immersion into propane at 85 K.