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A simple method for the handling and orientation of small specimens for electron microscopy
Author(s) -
Carson J.,
Lee R. M. K. W.,
Forrest J. B.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00373.x
Subject(s) - glutaraldehyde , fixation (population genetics) , electron microscope , lens (geology) , microscopy , materials science , optics , computer science , biology , chemistry , chromatography , physics , biochemistry , gene
SUMMARY Cyanoacrylic glue (Eastman 910) was used to affix small pieces of nasal scrapings to lens paper immediately before fixation in the glutaraldehyde. The lens paper not only served to hold specimens together so that they were not lost during tissue processing, but also functioned as a ‘landmark’ for the specimens, so that specimens could be oriented in a specific manner during embedding and subsequent sectioning.