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Protein‐A gold particles as markers in replica immunocytochemistry: high resolution electron microscope investigations of plasma membrane surfaces
Author(s) -
Mannweiler K.,
Hohenberg H.,
Bohn W.,
Rutter G.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00363.x
Subject(s) - immunocytochemistry , electron microscope , replica , resolution (logic) , plasma , membrane , scanning electron microscope , materials science , electron , high resolution , chemistry , biophysics , optics , biology , physics , biochemistry , computer science , composite material , art , artificial intelligence , visual arts , endocrinology , remote sensing , quantum mechanics , geology
SUMMARY Due to their high atomic number contrast in transmission electron microscopy, gold particles are ideal markers in surface replicas of cultured cells. The suitability of protein‐A‐coated gold particles in replica immunocytochemistry for labelling surface antigens is demonstrated using measles virus‐infected cells as a model system. Labelled areas can easily be distinguished from unlabelled areas, and even markers positioned in the evaporation shadow of large structures can be accurately identified, which is a prerequisite for an exact quantification and mapping of antigen. In addition, the ultrastructure of labelled areas can still be visualized because of the small size of the marker.