Premium
Energy dispersive X‐ray microanalysis of air dried casein micelles from bovine milk
Author(s) -
O'Brien Timothy L.,
Baumrucker Craig R.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb02492.x
Subject(s) - distilled water , glutaraldehyde , microanalysis , micelle , chemistry , casein , chromatography , aqueous solution , aqueous two phase system , colloid , scanning electron microscope , materials science , food science , organic chemistry , composite material
SUMMARY Secretory proteins (casein micelles) were air dried prior to elemental analysis either without treatment, following glutaraldehyde fixation and dialysis, or following dialysis against distilled water. Samples were then examined by energy dispersive X‐ray microanalysis in a scanning transmission electron microscope (STEM). Untreated samples and samples dialysed against distilled water retain colloidal (bound) elements as compared to glutaraldehyde treated samples. Contamination of air dried casein micelles by ionic constituents in the non‐protein aqueous phase was observed in the untreated sample and in the glutaraldehyde treated sample. Dialysis of casein micelles against distilled water reduced contamination from the aqueous phase without diminishing the apparent concentrations of colloidal elements.