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Photoelectron microscopy of cell surface topography
Author(s) -
Nadakavukaren Karen K.,
Rempfer Gertrude F.,
Griffith O. Hayes
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01270.x
Subject(s) - micrograph , electron micrographs , transmission electron microscopy , microscopy , materials science , x ray photoelectron spectroscopy , electron microscope , scanning electron microscope , optics , chemistry , nanotechnology , composite material , nuclear magnetic resonance , physics
SUMMARY Photoelectron micrographs of gold‐palladium coated mouse 3T3 cells and chick embryo fibroblasts are presented. Since the gold‐palladium suppresses differences in work function, the cell morphology seen in these micrographs is due to relief contrast. The heights of comparable cells were measured from the parallax present in transmission electron micrograph stereo‐pairs of cell surface replicas. The origin of relief contrast and the effect of cell surface relief on the working depth of field in photoelectron images of cells are discussed. The micrographs demonstrate that photoelectron microscopy is very sensitive to fine details of cell surface topography, and that the working depth of field is not a limiting factor in the imaging of well‐spread tissue culture cells.