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Strong evidence against section thawing whilst cutting on the cryo‐ultratome
Author(s) -
Frederik P. M.,
Busing W. M.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01262.x
Subject(s) - thin section , ice crystals , section (typography) , materials science , melting temperature , frozen section procedure , geology , mineralogy , composite material , optics , physics , pathology , medicine , advertising , business
SUMMARY The distribution of ice crystal sizes in frozen thin sections correlates with the size distribution in freeze fracture replicas. In the hypothetical case of melting (and refreezing) during cyro‐sectioning this correlation should be lost as was found after intentional melting or frozen thin sections. These observations demonstrate that there is neither a through section melting nor a growth of ice crystals during cryo‐ultramicrotomy.