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A technique for obtaining ribbons of epoxy and other plastic sections for light microscope histology
Author(s) -
Richards Peter R.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01261.x
Subject(s) - epoxy , materials science , polyethylene glycol , composite material , electron microscope , scanning electron microscope , optical microscope , microscope , microscopy , optics , chemical engineering , physics , engineering
SUMMARY A method is described by which ribbons of thick, large area sections of material embedded in epoxy resins prepared from standard recipes for electron microscopy, can be cut using conventional microtomes. The epoxy blocks are double embedded in an epoxy/polyethylene glycol mixture and ribbons are cut dry with fluorocarbon coated long‐edged (‘Ralph’) glass knives. The method can also be applied to other plastic embedding media such as glycol methacrylate.