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Sectioning and imaging hard mineral fibres in biological tissues
Author(s) -
Johnson N. F.,
Ibe K.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01245.x
Subject(s) - micrometer , mineral , uranyl acetate , materials science , biological specimen , mineral deposit , diamond , optical sectioning , chemistry , optics , microscopy , composite material , geology , metallurgy , electron microscope , physics , geochemistry
SUMMARY A technique is described which overcomes the problems associated with sectioning biological tissue containing hard mineral fibres. 0·2–0·5 μm thick sections were cut with a diamond knife, placed in a folding grid, conventionally stained with uranyl acetate and lead citrate and viewed at an accelerating voltage of 200 kV in the scanning transmission mode.