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Resolution in light microscope autoradiography using a carbon‐14 labelled line source
Author(s) -
Vanroelen Ch.,
Harrisson F.,
Andries L.,
Vakaet L.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01236.x
Subject(s) - resolution (logic) , microscope , electron microscope , optics , normalization (sociology) , emulsion , line (geometry) , materials science , microscopy , optical microscope , photographic emulsion , electron density , scanning electron microscope , chemistry , analytical chemistry (journal) , electron , physics , geometry , mathematics , chromatography , biochemistry , quantum mechanics , artificial intelligence , sociology , computer science , anthropology , silver halide
SUMMARY Light microscope autoradiographs were prepared from a 14 C line source. Two factors that affect resolution were studied: emulsion thickness and section thickness. The distribution around the line source was determined using the half‐distance (HD) value to quantify the resolution. An increase in HD value was observed with thicker sections or emulsion layers. The shape of the curve reflecting the grain density distributions around these line sources was very similar. After normalization in HD and relative grain density units, an average distribution was calculated and compared with the shape of normalized density distributions obtained from electron microscope autoradiographs. Other than a discrepancy near the source, an acceptable correlation was observed.