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The use of a simple method to avoid cell shrinkage during SEM preparation
Author(s) -
Wollweber Leo,
Stracke Roland,
Gothe Uwe
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01211.x
Subject(s) - shrinkage , glutaraldehyde , tannic acid , uranyl acetate , scanning electron microscope , uranyl , materials science , chemistry , chemical engineering , nuclear chemistry , chromatography , electron microscope , composite material , optics , metallurgy , organic chemistry , physics , uranium , engineering
SUMMARY In the course of preparing specimens for scanning electron microscopy, both glutaraldehyde and OsO 4 ‐fixed cells exhibit a considerable shrinkage with a reduction of the mean cellular diameter of about 45% after critical point drying. However, if cells are successively treated with glutaraldehyde, OsO 4 , tannic acid and uranyl acetate solutions, cellular shrinkage of only 5% is observed.